Composite

Part:BBa_K1878002:Design

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


pBAD/His/iRFP670


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2271
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2271
    Illegal NheI site found at 1260
    Illegal NheI site found at 1415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2271
    Illegal BglII site found at 1329
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1316
    Illegal XhoI site found at 1325
    Illegal XhoI site found at 1884
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2271
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2271
    Illegal NgoMIV site found at 1464
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1970
    Illegal BsaI site found at 2450
    Illegal BsaI.rc site found at 1527
    Illegal SapI site found at 961


Design Notes

The PstI site within the reporter has been removed. There are several BsaI sites within the protein so do not use the BsaI enzyme for Golden Gate DNA Assembly.



Source

iRFP670 is a mutated form of the Rhodopseudomonas palustris bacterial phytochrome photoreceptor.

References